Reporter

Part:BBa_K1878001

Designed by: John Luke Garcia   Group: iGEM16_Rice   (2016-10-14)


iRFP713 (the fluorescent protein formerly known as "iRFP")

General Properties

This fluorescent protein has an excitation wavelength of 690 nm and an emission wavelength of 713 nm. The extinction coefficient is 98000 M-1 cm-1. Its quantum yield is 6.3%. Its photostability half-life is 960 seconds. Its pKa is 4.5. Near-infrared fluorescent proteins are good for long-term in vivo imaging and exhibit high brightness in bacteria, mammalian cells, and mice.

The original citation is as follows: The original citation is as follows: D.M. Shcherbakova and V.V. Verkhusha. "Near-infrared fluorescent proteins for multicolor in vivo imaging." Nature Methods, 2013. 10(8): p. 751-4.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 10
    Illegal XhoI site found at 346
    Illegal XhoI site found at 352
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 127
    Illegal NgoMIV site found at 609
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None